CEBPA resembles Roman god Janus.

The CCAAT/enhancer binding protein, encoded by the CEBPA gene, is a transcription factor that induces the expression of genes involved in terminal differentiation of granulocytes. Recently, CEBPA mutations have been added to the list of single-gene mutations that may define a separate disease entity in cytogenetically normal acute myeloid leukemia (CNAML), which is increasingly recognized as a molecularly heterogeneous disease.1 Apart from CEBPA mutations, mutations in nucleophosmin (NPM1), in FMS-related tyrosine kinase 3 (FLT3), and in the Wilms tumor 1 (WT1) gene can be identified in CN-AML. In pediatric AML, the frequency of CN-AML is lower in comparison with adult AML and accounts for only 20% to 25% of cases. Given the differences in overall outcome between adult and pediatric AML, the molecular classification and subsequent treatment stratification of CN-AML needs to be validated in separate pediatric studies. For FLT3, NPM1, and WT1, such data are currently available.2-4 Different NPM1 mutations were found in pediatric as compared with adult AML, underlining the need for separate studies in pediatric AML3 In this issue, Ho et al screen a large series of pediatric AML samples, obtained from 3 Children’s Oncology Group (COG) studies, for CEBPA mutations by fragment-length analysis.5 Acquired mutations were identified in 4.5% of all patients and clustered in CN-AML. In fact, 17% of pediatric patients with CN-AML were CEBPA-mutated. The majority of patients (82%) had “double” mutations in both the N-terminal domain and in the bZIP domain of the CEBPA gene. Patients with CEBPAmutated AML showed similar survival rates as children with core-binding factor AML [ie, AML with inv(16) or t(8;21)]. No differences in outcome were detected between double and single mutants, which is in contrast to Wouters et al, who recently reported that favorable outcome is restricted to patients with “double mutations,” which are usually localized on different alleles, resulting in the absence of wild-type protein.6 Recent data also suggest that accompanying genetic abnormalities may influence the prognostic impact of CEBPA mutations. For instance, Reneville et al showed that favorable outcome was restricted to patients with CNAML without accompanying cytogenetic abnormalities or FLT3/ITD.7 Ho et al, however, found additional cytogenetic abnormalities in 14% of patients, with limited impact on outcome (overall survival 80% for patients with a normal karyotype, and 78% for all patients together). CEBPA, however, may contribute to leukemogenesis in opposite ways, which led us to compare CEBPA with the Roman god Janus. According to Roman mythology, he was the god of doors and gateways and is typically displayed as having 2 faces looking in opposite directions. His name is already permanently associated with the Janus kinases, which are characterized by an inhibitory and activating kinase domain and are involved in Down syndrome leukemias and T-cell acute lymphoblastic leukemia. At least